SARs stimulate but do not confer position independent gene expression.
Identifieur interne : 006566 ( Main/Exploration ); précédent : 006565; suivant : 006567SARs stimulate but do not confer position independent gene expression.
Auteurs : L. Poljak [Suisse] ; C. Seum ; T. Mattioni ; U K LaemmliSource :
- Nucleic acids research [ 0305-1048 ] ; 1994.
Descripteurs français
- KwdFr :
- ADN (), Animaux, Cellules HeLa, Chloramphenicol O-acetyltransferase (génétique), Composition en bases nucléiques, Drosophila (génétique), Expression des gènes, Gènes rapporteurs, Histone (génétique), Humains, Protéines du choc thermique HSP70 (génétique), Régions promotrices (génétique), Saccharomyces cerevisiae (génétique), Souris, Transcription génétique, Transfection, Virus simien 40 (génétique), Éléments activateurs (génétique).
- MESH :
- génétique : Chloramphenicol O-acetyltransferase, Drosophila, Histone, Protéines du choc thermique HSP70, Saccharomyces cerevisiae, Virus simien 40.
- ADN, Animaux, Cellules HeLa, Composition en bases nucléiques, Expression des gènes, Gènes rapporteurs, Humains, Régions promotrices (génétique), Souris, Transcription génétique, Transfection, Éléments activateurs (génétique).
English descriptors
- KwdEn :
- Animals, Base Composition, Chloramphenicol O-Acetyltransferase (genetics), DNA (chemistry), Drosophila (genetics), Enhancer Elements, Genetic, Gene Expression, Genes, Reporter, HSP70 Heat-Shock Proteins (genetics), HeLa Cells, Histones (genetics), Humans, L Cells (metabolism), Mice, Promoter Regions, Genetic, Saccharomyces cerevisiae (genetics), Simian virus 40 (genetics), Transcription, Genetic, Transfection.
- MESH :
- chemical , chemistry : DNA.
- chemical , genetics : Chloramphenicol O-Acetyltransferase, HSP70 Heat-Shock Proteins, Histones.
- genetics : Drosophila, Saccharomyces cerevisiae, Simian virus 40.
- metabolism : L Cells.
- Animals, Base Composition, Enhancer Elements, Genetic, Gene Expression, Genes, Reporter, HeLa Cells, Humans, Mice, Promoter Regions, Genetic, Transcription, Genetic, Transfection.
Abstract
Two minimal scaffold-associated regions (SARs) from Drosophila were tested in stably transformed cells for their effects on the expression of reporter genes. The expression of genes bounded by two SARs is consistently stimulated by about 20- to 40-fold, if the average of a pool of cell transformants is analyzed. However, analysis of individual, stable cell transformants demonstrates that flanking SAR elements do not confer position-independent expression on the reporter gene and that the extent of position-dependent variegation is similarly large with or without the flanking SAR elements. The SAR stimulation of expression is observed in stable but not in transiently transfected cell lines. The Drosophila scs and scs' boundary elements, which do not bind to the nuclear matrix in vitro, are only about one-tenth as active as SARs in stimulating expression in stable transformants. Interestingly, the SAR stimulatory effect can be blocked by a fragment containing CpG islands (approximately 70% GC), if positioned between the SAR and the enhancer. In contrast, when inserted in the same position, control fragments, such as the scs/scs' elements, do not interfere with SAR function.
DOI: 10.1093/nar/22.21.4386
PubMed: 7971269
Affiliations:
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Poljak</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular Biology, University of Geneva, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Molecular Biology, University of Geneva</wicri:regionArea><placeName><settlement type="city">Genève</settlement><region nuts="3" type="region">Canton de Genève</region></placeName><orgName type="university">Université de Genève</orgName></affiliation></author><author><name sortKey="Seum, C" sort="Seum, C" uniqKey="Seum C" first="C" last="Seum">C. Seum</name></author><author><name sortKey="Mattioni, T" sort="Mattioni, T" uniqKey="Mattioni T" first="T" last="Mattioni">T. Mattioni</name></author><author><name sortKey="Laemmli, U K" sort="Laemmli, U K" uniqKey="Laemmli U" first="U K" last="Laemmli">U K Laemmli</name></author></analytic><series><title level="j">Nucleic acids research</title><idno type="ISSN">0305-1048</idno><imprint><date when="1994" type="published">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Composition</term><term>Chloramphenicol O-Acetyltransferase (genetics)</term><term>DNA (chemistry)</term><term>Drosophila (genetics)</term><term>Enhancer Elements, Genetic</term><term>Gene Expression</term><term>Genes, Reporter</term><term>HSP70 Heat-Shock Proteins (genetics)</term><term>HeLa Cells</term><term>Histones (genetics)</term><term>Humans</term><term>L Cells (metabolism)</term><term>Mice</term><term>Promoter Regions, Genetic</term><term>Saccharomyces cerevisiae (genetics)</term><term>Simian virus 40 (genetics)</term><term>Transcription, Genetic</term><term>Transfection</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ()</term><term>Animaux</term><term>Cellules HeLa</term><term>Chloramphenicol O-acetyltransferase (génétique)</term><term>Composition en bases nucléiques</term><term>Drosophila (génétique)</term><term>Expression des gènes</term><term>Gènes rapporteurs</term><term>Histone (génétique)</term><term>Humains</term><term>Protéines du choc thermique HSP70 (génétique)</term><term>Régions promotrices (génétique)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Souris</term><term>Transcription génétique</term><term>Transfection</term><term>Virus simien 40 (génétique)</term><term>Éléments activateurs (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chloramphenicol O-Acetyltransferase</term><term>HSP70 Heat-Shock Proteins</term><term>Histones</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Drosophila</term><term>Saccharomyces cerevisiae</term><term>Simian virus 40</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chloramphenicol O-acetyltransferase</term><term>Drosophila</term><term>Histone</term><term>Protéines du choc thermique HSP70</term><term>Saccharomyces cerevisiae</term><term>Virus simien 40</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>L Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Composition</term><term>Enhancer Elements, Genetic</term><term>Gene Expression</term><term>Genes, Reporter</term><term>HeLa Cells</term><term>Humans</term><term>Mice</term><term>Promoter Regions, Genetic</term><term>Transcription, Genetic</term><term>Transfection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN</term><term>Animaux</term><term>Cellules HeLa</term><term>Composition en bases nucléiques</term><term>Expression des gènes</term><term>Gènes rapporteurs</term><term>Humains</term><term>Régions promotrices (génétique)</term><term>Souris</term><term>Transcription génétique</term><term>Transfection</term><term>Éléments activateurs (génétique)</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two minimal scaffold-associated regions (SARs) from Drosophila were tested in stably transformed cells for their effects on the expression of reporter genes. The expression of genes bounded by two SARs is consistently stimulated by about 20- to 40-fold, if the average of a pool of cell transformants is analyzed. However, analysis of individual, stable cell transformants demonstrates that flanking SAR elements do not confer position-independent expression on the reporter gene and that the extent of position-dependent variegation is similarly large with or without the flanking SAR elements. The SAR stimulation of expression is observed in stable but not in transiently transfected cell lines. The Drosophila scs and scs' boundary elements, which do not bind to the nuclear matrix in vitro, are only about one-tenth as active as SARs in stimulating expression in stable transformants. Interestingly, the SAR stimulatory effect can be blocked by a fragment containing CpG islands (approximately 70% GC), if positioned between the SAR and the enhancer. In contrast, when inserted in the same position, control fragments, such as the scs/scs' elements, do not interfere with SAR function.</div></front></TEI><affiliations><list><country><li>Suisse</li></country><region><li>Canton de Genève</li></region><settlement><li>Genève</li></settlement><orgName><li>Université de Genève</li></orgName></list><tree><noCountry><name sortKey="Laemmli, U K" sort="Laemmli, U K" uniqKey="Laemmli U" first="U K" last="Laemmli">U K Laemmli</name><name sortKey="Mattioni, T" sort="Mattioni, T" uniqKey="Mattioni T" first="T" last="Mattioni">T. Mattioni</name><name sortKey="Seum, C" sort="Seum, C" uniqKey="Seum C" first="C" last="Seum">C. Seum</name></noCountry><country name="Suisse"><region name="Canton de Genève"><name sortKey="Poljak, L" sort="Poljak, L" uniqKey="Poljak L" first="L" last="Poljak">L. Poljak</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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